Manipulation of public massive ChIPseq and methylome datasets using R
Transcription factors (TFs) are sequence-specific binding proteins controlling the spatiotemporal expression of genes. Since the genomic occupancy of TF is highly dynamic, it is crucial to study TF binding sites (TFBS) in a cell-specific and in vivo context.
This workshop will focus on the manipulation of public cistrome and methylome datasets recorded in MethMotif and TFregulomeR databases, using R packages. These processes greatly facilitate the manipulation and analysis of TFBS and methylome meta-data, in particular for the characterisation of TF binding partners and cell-specific TFBS, along with the study of TF functions depending on their association with other TFs and/or on DNA methylation levels.
– Basic knowledge of R language.
– Attendees must bring their own computer with R or RStudio installed.