The CSI FACS Facility led by A/Prof Motomi Osato was established in 2008 to cater to the flow cytometry needs of CSI and other departments located on the NUS Kent Ridge campus. We have since expanded our customer base to include external customers from DSO and NTU. With globally-accredited certified cytometrists as the key operators, the facility aims to provide users with access to state-of-the-art flow cytometry equipment, advice on flow cytometry experiment set-up, sample preparation tips, as well as rental of flow cytometry software for data analysis.
Located in MD6, the laboratory houses two BD FACSAria cell sorters and one BD LSRII analyser equipped with multiple lasers for simultaneous measurement of up to 16 parameters. The operators are experienced in running several flow cytometry applications including immunophenotyping, cell cycle, apoptosis, cell proliferation, stem cell side population, detection of reactive oxygen species (ROS) and calcium flux. The staff actively work with new users to provide training and guidance on how to analyze and interpret their flow cytometry data. They also help to troubleshoot and provide advice to users on how to improve their experiments.
Fluorescence activated cell sorting (FACS) is a cell isolation technique renowned for its high purity, and has proven to be useful to singularly sort cells into 96 well plate for clone selection, drug screening assays or single-cell RNA analysis. It has also been sought after as a superior alternative to long-standing practices like colony picking. The cells can also be sorted onto a glass slide for further microscopy work. The CSI FACS facility has successfully sorted cells for transplantation into animal models, microarrays, as well as chromatin immunoprecipitation (ChIP) experiments. As CSI now serves as RNA Biology Centre, the facility has started using RNA detection systems by flow cytometry such as PrimeFlow RNA assay which allows for flow-based RNA in situ hybridization with single cell resolution.