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Transgenic and Gene Targeting Core

The Transgenic & Gene Targeting Facility of CSI Singapore is established in 2009 under the leadership of Dr. Md Zakir Hossain. The facility offers comprehensive scientific services and collaborations for NUS research laboratories as well as other academic and biotech industries in Singapore and globally.

The facility is equipped with the state-of-the-art micro-manipulation station to perform embryonic stem (ES) cell injection, pronuclear DNA/RNA/protein injection and intracytoplasmic sperm injection (ICSI). It exploits mammalian embryogenesis and performs genetic manipulation in ES cells and pronuclear embryos for the generation of transgenic, chimera, knockout and knockin mouse models. We routinely perform procedures of embryo harvesting, in-vitro-fertilization (IVF), micro-injection and embryo transfer techniques to generate novel mouse model as well as cryo-preservation and rederivation of mouse line from cryo-preserved germplasms (e.g., sperm, egg and embryo). With the advent of cutting edge technology (CRISPR/Cas), the facility is now experiencing greater strength ever to satisfy the ambitious demands for genetic manipulation in diverse species (e.g., mice and rats) and strains (e.g., C56BL/6 and FVB/N) to create indel, large deletion and insertion in the genome with cost and time effective manner. It is possible to generate C57BL/6 knockout mice (F0-breeders) in 3 months’ time with cost less than S$10,000. More than twenty-five Principal Investigators (PIs) from NUS, DUKE-NUS, TLL, NCCS and A*STAR have received their service and collaborative supports.

“When we asked Dr. Md Zakir Hossain to make conventional knock-in mouse of R122C, he was able to successfully produce it. In addition, Dr. Zakir has made flox allele of ARID1A and knock-in of Claudin18-Cre for us. Transgenic eR1-Cre mouse produced by Dr. Zakir is transforming my lab to stem cell studies. Most recently. Dr. Zakir successfully made knock-in of flox mouse of R122C by using CRISPR technology. Needless to say but we are very happy to have cooperated with Dr. Zakir and the Transgenic & Gene Facility is indeed a very valuable facility in CSI.”

– Prof Yoshiaki Ito, Senior Principal Investigator from Cancer Science Institute of Singapore

“We have been using Dr Zakir Hossain’s facility to generate several mice strains for our work. Dr Hossain and his team have been extremely helpful, discussing with us the projects at the outset, and continually update us on the progress. They also work with us to trouble shoot when necessary, till we obtain our desired strains of mice. It has been a great experience working with them, which has made us go back to them for our further work.”

– Prof Kanaga Sabapathy, Principal Investigator at National Cancer Centre Singapore (NCCS)

transgenic-core

The Transgenic and Gene Targeting Facility Members: Yew Yunn Cheng, Shi Jizhong, Yu Shuizhou and Dr Zakir Hossain (left to right)

The Transgenic and Gene Targeting Facility is looking forward to work more closely with PIs to be part of the research projects using in-vivo models: i) to address novel genetic mechanism through sophisticated genome manipulations in-vivo; ii) to develop mouse model for human diseases leading to identification of novel disease pathways; and iii) to establish pre-clinical animal model leading to discovery and development of novel therapeutic modalities.